![]() ![]() The pest becomes the adult from pupa after four molting stages of larvae (Venkatesha 2006), and both larvae and adults are known to eat the epidermal tissues of vegetable leaves greedily. It is one of the most destructive pests found in Japan, China, India, and other countries and causes considerable economic damage, especially in vegetable cultivation (Wang et al. The ladybird beetle Henosepilachna vigintioctopunctata is an agricultural pest, since the insect exclusively feeds on leaves of Solanaceae crops such as potato, tomato, eggplant, and so on. Thus, RNA molecules having such a function can be called RNA-based pesticides or RNA insecticides (Palli 2014 Gu and Knipple 2013). When RNA interference (RNAi) by double-stranded RNA (dsRNA) is induced with respect to a gene essential for the growth of a target pest, expression of that gene is suppressed, and, as a result, the growth of the pest is inhibited. Recently, RNA has attracted attention as a potential candidate for pest control. Thus, the development of environmentally-friendly pest control methods that replace conventional chemical pesticides is desired and required. Although they have brought great benefits to crop cultivation, they also have various undesirable environmental consequences, for example, effects on beneficial insects and soil microbes and the emergence of pesticide-resistant pests (Woodcock et al. Many powerful chemical pesticides have been used to combat pests in agriculture. ![]() These results suggest that this system is capable of producing stabilized dsRNA for RNAi at low cost, and it will open a way to practical application of dsRNA as an environmentally-friendly agricultural insecticide. vigintioctopunctata, diap1 expression in the pest was suppressed, and the leaf-feeding activity of the larvae declined. When the sterilized microbes containing diap1*-dsRNA were fed to larvae of H. In addition, we developed a convenient method for stabilizing the dsRNA within the microbes by simply sterilizing the diap1*-dsRNA-expressing C. glutamicum by convergent transcription using a strong promoter derived from corynephage BFK20, and the amount of dsRNA accumulated in fermented cells reached about 75 mg per liter of culture. ![]() vigintioctopunctata, using an industrial strain of Corynebacterium glutamicum as the host microbe. We constructed an efficient production system for diap1*-dsRNA, which suppresses expression of the essential gene diap1 (encoding death-associated inhibitor of apoptosis protein 1) in H. In this study, we selected a ladybird beetle, Henosepilachna vigintioctopunctata, as a model target pest that devours vegetable leaves, and examined the effects of feeding the pest sterilized microbes highly accumulating a target dsRNA for RNAi induction. Double-stranded RNA (dsRNA) inducing RNA interference (RNAi) is expected to be applicable to management of agricultural pests. ![]()
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